Part:BBa_K4421027
CAR_library
As we have mentioned in BBa_K4421003, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on BBa_K4421003.
Structure of CAR_library
Our design is based on second-generation CAR, consisting of 1)endodomain: CD28 and CD3ζ 2)transmembrane domain: CD8TM 3)ectodomain: CD8 hinge and the scFv library. The unique point of our design is the scFv library, theoretically composed of all kinds of scFvs which can be generated by human bodies. In our cellular experiment, we selected 10 scFvs to form a small library, which is detailed in BBa_K4421003.
Theoretical base
According to the clonal selection theory, a healthy adult with a normal immune system could produce a very large number of antibodies against all antigens beforehand, including heterogeneous tumor antigens. So we can utilize these antibodies to form an antibody or scFv library which aims for almost all antigens.
Special Note
Since our part BBa_K4421003 is a general concept without a specific sequence, we use cetuximab scfv (BBa_K4421011) to serve as a placeholder. In other words, the place where BBa_K4421011 at can be replaced by any other scFv to form diverse CAR constructs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2042
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 298
Illegal NgoMIV site found at 1832
Illegal NgoMIV site found at 1898
Illegal NgoMIV site found at 2065 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1439
Illegal SapI.rc site found at 1996
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